PURIFICATION OF RECEPTOR PROTEINS IN THE NITRIC OXIDE SIGNALING PATHWAY

picture of Ethan Paddock presenting his/her poster: PURIFICATION OF RECEPTOR PROTEINS IN THE NITRIC OXIDE SIGNALING PATHWAY

Ethan Paddock , Jacob Milton, Nathaniel Jim, Rachelle Eddie, Matthew J. Gage PhD

PURIFICATION OF RECEPTOR PROTEINS IN THE NITRIC OXIDE SIGNALING PATHWAY

The process of angiogenesis (the growth of new blood vessels) is a common step during development, wound healing, and tissue damage.  Angiogenesis is also one of the 6 hallmarks associated with tumor growth. Regulation of angiogenesis involves several different protein-protein interactions.  This study focuses on three interacting proteins: CD47, TSP-1, and VEGFR-2, which are all involved in Nitric Oxide (NO) signaling.  The IG domain of CD47 has been shown to interact with TSP-1, inhibiting NO production and preventing angiogenesis. It was recently shown that VEGFR-2 associates with CD47 to upregulate NO production and this interaction can be disrupted by TSP-1. The nature of TSP-1, CD47, and VEGFR-2 interactions are not fully understood. The goal of this project is to characterize the molecular interactions between CD47, TSP-1, and VEGFR-2. The first step of this project is to establish expression and purification conditions for these proteins. That work is presented here.

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