A NEW TREATMENT FOR BIOSOLIDS TO IMPROVE SUSTAINABILITY USING RECOVERY OF HELMINTH OVA, ENTERIC VIRUSES, AND COLIFORMS AS A MEASURE OF EFFICACY

Alejandro Badilla , Posey, Jesse, Gerba, P. Charles PhD, Tamimi, Akrum PhD

A NEW TREATMENT FOR BIOSOLIDS TO IMPROVE SUSTAINABILITY USING RECOVERY OF HELMINTH OVA, ENTERIC VIRUSES, AND COLIFORMS AS A MEASURE OF EFFICACY

 

Treating wastewater sludge to U.S. EPA Class A level is usually expensive and requires a great amount of energy and / or time.  The MagnaGro Process™ eradicates enteric viruses, fecal coliform bacteria and Ascaris eggs by increasing the pH of the sludge and by mixing the sludge with an agricultural fumigant.  The process takes 24 hours and requires no energy and is low cost. In order to prove that the process meets the U.S. EPA requirements, wastewater sludge is usually inoculated with enteric viruses and Ascaris suum eggs and the sludge is treated using the MagnaGro Process™.  Samples are taken after the inoculation but before the start of the treatment and at the end of the treatment process (24 hours) to test for enteric viruses, fecal coliform bacteria and helminth (Ascaris) ova. 

Each experiment was done in triplicates in order to ensure accurate representations of the data. The method of sludge treatment was tested for efficacy and to find a log reduction to classify the biosolids as class A or class B. The initial spiked biosolid was processed by EPA enteric virus recovery protocol and a modified version of EPA protocol for Ascaris ova recovery. Recovery of near-initial amounts of enteric virus (represented by poliovirus LSC-1) and Ascaris ova were determined from 0-hour samples. It was determined that after 24 hours, a complete log reduction of enteric virus was found and no virus was recovered. Also, the log reductions were met with the coliforms and Ascaris ova, classifying these biosolids as class A and proving efficacy of MagnaGro Process™ implementation.

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