NOVEL NON-INJURY VERSUS INJURY MIGRATION ASSAYS

Kaitlyn Ammann , Katrina Decook, Phat L. Tran, Marvin J. Slepian

NOVEL NON-INJURY VERSUS INJURY MIGRATION ASSAYS

Cellular migration is important when considering many tissue-engineering strategies, and it plays a vital role in vascular remodeling, wound healing, and tissue repair. Quantification of cellular migration can provide important information about cellular activity as cells interact with each other and with
surfaces. Common assays for quantifying migration injure cells and potentially influence migration. In this study, two simple, scalable non-injury migration assays: 1) hollow cylinder for out-migration and 2) polydimethylsiloxane (PDMS) lift-off stamp for in-migration, were developed to study the effects of different ECM surfaces on smooth muscle cell (SMC) and human umbilical vascular endothelial cell (HUVEC) migration in comparison to the commonly used scrape-wound injury model. Here, we found optimal adhesion and highest migration rate for SMCs and HUVECs on Collagen IV. The rate of migration over time for HUVECs and SMCs under the scrape-wound injury assay is 0.30 and 0.35, respectively. For HUVECs, the percent of injury migration is 6% and 27% (p < 0.05) higher than lift-off and hollow cylinder assays, accordingly. Meanwhile, injured SMCs migrate 23% faster than lift-off assay (p < 0.05) but insignificant with hollow cylinder at 1% (p < 0.05).

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