POLLEN TUBE GUIDANCE TOWARDS UNFERTILIZED ARABIDOPSIS THALIANA MYB98 MUTANT OVULES IS ALSO REDUCED IN A MICROSYSTEM-BASED GUIDANCE ASSAY

picture of Salika Dunatunga presenting his/her poster: POLLEN TUBE GUIDANCE TOWARDS UNFERTILIZED ARABIDOPSIS THALIANA MYB98 MUTANT OVULES IS ALSO REDUCED IN A MICROSYSTEM-BASED GUIDANCE ASSAY

Salika Dunatunga , Matt Stamm, Linan Jiang, Yitshak Zohar, Ravishankar Palanivelu

POLLEN TUBE GUIDANCE TOWARDS UNFERTILIZED ARABIDOPSIS THALIANA MYB98 MUTANT OVULES IS ALSO REDUCED IN A MICROSYSTEM-BASED GUIDANCE ASSAY

Previous publications of a tested semi-in vivo microsystem-based assay quantified wild-type pollen tubes growth towards the microchannel chambers containing unfertilized ovules versus empty chambers at a ratio of 2.7:1. In this follow-up experiment, the Arabidopsis thaliana mutant myb98 is used to confirm the failure of pollen tube attraction as the basis of reduced seed-set in myb98 plants. The MYB98 transcription factor has been shown in a previous study to induce the expression of proteins localized in the filiform apparatus of the synergid cell in the ovule and is thus theorized to play a role in pollen tube attraction. Unfertilized ovules from male sterile (ms1) plants were used as a control and a ratio of 3.3:1 pollen tubes from LAT52:GFP pollen entering the ovule chamber versus the empty chamber was achieved. However, with unfertilized myb98 ovules, a ratio of only 1.3:1 was achieved. These results confirm that myb98 ovules are deficient in pollen tube attraction and establish the utility of our microsystem-based guidance assay in analyzing Arabidopsis thaliana pollen tube-ovule interactions. Supported in part by funding from the UA Provost's Office.

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