IQGAP1 PLAYS A CRITICAL ROLE IN CELL REPAIR AFTER ACUTE KIDNEY INJURY

picture of Eva Nichols presenting his/her poster: IQGAP1 PLAYS A CRITICAL ROLE IN CELL REPAIR AFTER ACUTE KIDNEY INJURY

Eva Nichols , Michael Lien, Dr. Kim Yong, Dr. Liwen Lai

IQGAP1 PLAYS A CRITICAL ROLE IN CELL REPAIR AFTER ACUTE KIDNEY INJURY

IQGAP1 is a multifunctional protein that binds to actin and is implicated with cytoskeleton remodeling, cell migration, cell polarity, cell-cell adhesion, and proliferation. IQGAP1 knock-out (KO) mice were used to evaluate their ability to recover from acute kidney injury (AKI). When confronted with ischemic reperfusion injury, KO mice exhibited a significantly impaired ability to proliferate its cells and rebuild the cytoskeleton as compared to similarly treated wild-type mice (WT), demonstrating a failure to overcome acute kidney injury due to lapses in cytoskeleton integrity. Immunofluorescence staining was performed using a proliferation marker Ki67. Degree of renal damage was evaluated with histopathological acute tubular necrosis (ATN) scoring of sections and serum creatinine and blood-urea nitrogen (BUN) levels, markers for renal function. The failure of KO mice to recover from kidney injury was confirmed by their higher ATN score along with higher levels of BUN and creatinine, indicating impaired renal function. In contrast, WT mice start to recover as early as two days after injury. Therefore, we conclude that IQGAP1 is an important gene for cell repair and proliferation after acute kidney injury. IQGAP1 may serve as a drug target for developing treatment for AKI.

This research is supported by NIH (grant number R01DK082718) and NSF WAESO. 

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