THE ROLE OF G-QUADRUPLEX INTERACTIONS ON PROTEIN EXPRESSION WITHIN THE BCL-2 PROMOTER.

picture of Nicholas Zehrbach presenting his/her poster: THE ROLE OF G-QUADRUPLEX INTERACTIONS ON PROTEIN EXPRESSION WITHIN THE BCL-2 PROMOTER.

Nicholas Zehrbach , Danzhou Yang, Megan Carver, Frank Li

THE ROLE OF G-QUADRUPLEX INTERACTIONS ON PROTEIN EXPRESSION WITHIN THE BCL-2 PROMOTER.

G-quadruplexes are recently discovered DNA secondary structures that have interested researchers as potential therapeutic targets against cancer. Gene promoters can contain segments of DNA that form G-quadruplexes, which, if stabilized, can inhibit or elevate transcription of gene products. The goal of this experiment was to study the G-quadruplex quadruplex formed in the Bcl2 promoter region. The P1 and Pu39 regions in the 360-bp Bcl-2 promoter can exhibit G-quadruplex formation. Multiple mutated versions of a vector with the Bcl-2 promoter, which controlled expression of a firefly luciferase gene, were transfected into HeLa cells. These cells were exposed to two isomer drugs, TMPyP2 and TMPyP4, which are either non-interactive or function as stabilizers that bind to G-quadruplexes. The protein expression of the vector was then measured using a luciferase assay and normalized to wild-type expression levels. These results suggest that removal of either of the two quadruplexes in the Bcl-2 promoter region negatively affects expression. However, the data also shows that there are transcription factors and other control elements in our promoter sequence which may influence our data in ways that have not been accounted for, and further research into the mutations is necessary. This grant was funded by #52006942 and R01GM083117.

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