REGENERATIVE POTENTIAL OF ADIPOSE AND CORD TISSUE DERIVED MESENCHYMAL STEM CELLS

picture of Jorge Alvarez presenting his/her poster: REGENERATIVE POTENTIAL OF ADIPOSE AND CORD TISSUE DERIVED MESENCHYMAL STEM CELLS

Jorge Alvarez , Mahmood S. Choudhery

REGENERATIVE POTENTIAL OF ADIPOSE AND CORD TISSUE DERIVED MESENCHYMAL STEM CELLS

Stem cell therapies can provide an alternative approach for repair and regeneration of tissues and organs. Mesenchymal stem cells (MSCs) are promising candidates for cell based therapies. Although bone marrow derived MSCs (BM-MSCs) have multi-lineage differentiation potential, it is not a preferred stem cell source because of the isolation process and low yield. The goal of this work is to evaluate the regenerative potential of human MSCs from two other sources: umbilical cord tissue and adipose tissue.  We are also investigating the effect of harvest-site, sex, age and health status on the regenerative potential of MSCs. Cells from each tissue were isolated and characterized by flow cytometry using typical MSC markers. Growth characteristics were investigated by population doublings, saturation density and plating efficiency. MSCs derived from both types of tissues were assessed for differentiation potential qualitatively and quantitatively. In vitro assays for wound healing and angiogenesis were investigated by scratch and matrigel assays, respectively.  FACS analysis showed that cultured cells were positive for CD73, CD90 and CD105 while being negative for CD45.MSCs from all sources showed high proliferative potential and CFUs. After induction, MSCs efficiently differentiated into adipose, bone, cartilage and neuronal structures as determined with histochemistry, immunoflourescence and real time RT-PCR.  We conclude that MSCs can easily be obtained from human tissues and it appears that adipose and cord tissues are suitable sources of stem cells for potential use in regenerative medicine.

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