STEM CELL SEEDING ON SCAFFOLDS WITH PATTERNED SURFACES

picture of Hussein Magale presenting his/her poster: STEM CELL SEEDING ON SCAFFOLDS WITH PATTERNED SURFACES

Hussein Magale , Nicklaus Diggins, Kitsie Penick, John A. Szivek Ph.D

STEM CELL SEEDING ON SCAFFOLDS WITH PATTERNED SURFACES

Cartilage tissue engineering strategies could potentially return Osteoarthritis patients’ joints to their original undamaged state. Adipose-derived Stem Cells (ADSCs) were seeded on patterned polybutylene terephthalate (PBT) scaffolds. ADSCs were isolated from human infrapatellar fat. Cells were expanded using standard cell culture techniques. Three different categories of scaffolds were used; scaffolds with patterned surfaces, scaffolds with crosshatch surfaces and scaffolds with no roads. The scaffolds were placed horizontally and vertically in the 24-well plate to understand cartilage tissue development on them. The cells were grown on scaffolds for three weeks. Scanning Electron Microscopy (SEM) was utilized to image cell growth and to evaluate cell alignment on the scaffolds. The cells that were grown on the scaffolds were aligned along the strands/roads of the scaffolds. There were no cells observed on the scaffolds without roads because their morphology was too rough to view with the SEM. A clear distinction could not be noted on whether the scaffold placement vertically or horizontally in the 24-well plate affects the cell growth.

Funded by WAESO Grant # V12UR006/V2012ur0016 and partly by UBRP.

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