EXPRESSION OF DJ-1 IN RENAL ISCHEMIA REPERFUSION INJURY

Guanhui Wu , Liwen Lai, Kim-Chong Yong

EXPRESSION OF DJ-1 IN RENAL ISCHEMIA REPERFUSION INJURY

DJ-1 (PARK7) is a protector for neurons against oxidative stress and cell death and has been shown to be up-regulated by ischemia reperfusion injury (IRI) in brain. However its role in renal injury is unknown. Since oxidative stress is part of the pathogenesis of  renal IRI, we measured the DJ-1 protein level in IRI mice kidney. Mice were subjected to 30 min of bilateral clamping of renal pedicles, followed by reperfusion for 1, 2, 4, or 24 h. The protein samples were isolated from kidneys and the samples were analyzed using Western blotting analysis and imaged using both chemiluminescence and florescence methods. After quantification using ImageJ, we showed that DJ-1 was down-regulated by 50% at 1 h post-IRI and remained slightly down-regulated at 24 h post-IRI in comparison to healthy kidney. In brain, the up-regulation of DJ-1 was shown at 8 h post-IRI. Currently, we are performing more experiments to refine the time course of DJ-1 expression in renal IRI. Our results suggest that DJ-1 is abundantly expressed in both healthy and IRI kidneys and may play important role in pathogenesis of renal IRI. 

Grant support: NIH R01DK082718 (PI: Dr. Liwen Lai)

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